Quantal acetylcholine release induced by mediatophore transfection.
收藏PubMed Central1996-05-28 更新2026-05-02 收录
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https://pmc.ncbi.nlm.nih.gov/articles/PMC39222/
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资源简介：
Mediatophore is a protein of approximately 200 kDa able to translocate acetylcholine in response to calcium. It was purified from the presynaptic plasma membranes of the electric organ nerve terminals. Mediatophore is a homooligomer of a 16-kDa subunit, homologous to the proteolipid of V-ATPase. Cells of the N18TG-2 neuronal line are not able to produce quantal acetylcholine release. We show here that transfection of N18TG-2 cells with a plasmid encoding the mediatophore subunit restored calcium-dependent release. The essential feature of such a release was its quantal nature, similar to what is observed in situ in cholinergic synapses from which mediatophore was purified. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1996-05-28
