Dataset for paper "Outer membrane and phospholipid composition of the target membrane affect the antimicrobial potential of first- and second-generation lipophosphonoxins"

Raw data of electrical current recording of planar lipid bilayer treated with Lipophosphonoxins (LPPO). Two different LPPO molecules (DR5026 or DR6180) at 5, 2.5 or 1.25 µg/ml concentrations were tested. The data (about 70 hours) are organized into separated folders according to LPPO (DR5026 or DR6180) and its concentration (5.00, 2.50 or 1.25 µg/ml). The files in these subfolders are raw electrical recordings (electrical current in pA, TXT format) from the planar lipid bilayer. Sampling rate was 1 kHz; individual lines are separated by 1 ms. All presented data were recorded at 50 mV membrane potential.<br> The compressed file "<strong>Raw-current-data-DR5026-DR6180.zip</strong>" (428 MB) contains subfolders: 469MB datafolder_DR5026_1.25<br> 417MB datafolder_DR5026_2.50<br> 476MB datafolder_DR5026_5.00<br> 445MB datafolder_DR6180_1.25<br> 200MB datafolder_DR6180_2.50<br> 163MB datafolder_DR6180_5.00 The compressed file "<strong>Graphical-overview-of-Raw-current-data-DR5026-DR6180.zip</strong>" contains plots (in PNG image format, generated by Gnuplot 5.0) of raw data recordings together with highlighted manually selected parts. The table "<strong>Data-selection-table-DR5026-DR6180.csv</strong>" (CSV format) contains manually selected parts of the recording using QuB software. Please refer to QuB software manual for detailed description of the presented parameters. The selected data in this table were analyzed and presented in the publication (Látrová et al. 2021). Description of the experiment: A Teflon chamber was divided into two compartments connected by a circular aperture of approx. 0.5 mm in diameter. Planar lipid bilayers (black lipid membranes) were formed by painting a solution of 3% (w/v) 1,2-diphytanoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (Avanti Polar Lipids) in n-decane-butanol (9:1, v/v) across the hole. Both compartments contained 1 ml of 1M KCl, 10mM Tris, pH 7.4. The temperature was kept at 25 °C. LPPOs were added to the <em>cis</em> side of the membrane at a concentration of 5, 2.5 or 1.25 µg/ml. The membrane current was measured with Ag/AgCl electrodes with a constant applied voltage of 50 mV. The current signal was amplified with an LCA-200-100GV amplifier (Femto) and digitized with a KPCI-3108 or KPCI-3102 card (Keithley). The signal was processed with QuB software (Nicolai, C. &amp; Sachs, F. Solving ion channel kinetics with the QuB software. <em>Biophys. Rev. Lett.</em> 8, 191–211 (2013)).本数据集为经脂质膦氧苷（Lipophosphonoxins, LPPO）处理的平面脂质双层电流记录原始数据。本次测试了两种不同的LPPO分子（DR5026与DR6180），浓度分别设置为5、2.5或1.25 µg/ml。全部数据时长约70小时，按照LPPO种类（DR5026或DR6180）及其浓度（5.00、2.50或1.25 µg/ml）分为独立子文件夹。 各子文件夹内存储的是平面脂质双层的原始电生理记录文件，格式为TXT，电流单位为皮安（pA），采样率为1 kHz，每行数据间隔为1 ms。所有记录均在膜电位50 mV的条件下采集。 压缩文件**Raw-current-data-DR5026-DR6180.zip**（大小428 MB）内含以下子文件夹：datafolder_DR5026_1.25（469 MB）、datafolder_DR5026_2.50（417 MB）、datafolder_DR5026_5.00（476 MB）、datafolder_DR6180_1.25（445 MB）、datafolder_DR6180_2.50（200 MB）、datafolder_DR6180_5.00（163 MB）。 压缩文件**Graphical-overview-of-Raw-current-data-DR5026-DR6180.zip**内含原始数据的可视化图谱，格式为PNG，由Gnuplot 5.0生成，图谱中标注了人工选取的信号片段。 CSV格式文件**Data-selection-table-DR5026-DR6180.csv**包含使用QuB软件手动选取的记录片段相关信息，相关参数的详细说明请参阅QuB软件手册。本表格中选取的片段已被分析并发表于Látrová等人2021年的研究中。 实验装置采用特氟龙（Teflon）腔室，该腔室被直径约0.5 mm的圆形小孔分为两个独立缓冲液室。平面脂质双层（黑色脂质膜）通过在小孔处涂抹3%（w/v）1,2-二植烷酰基-sn-甘油-3-磷酸-(1'-rac-甘油)（Avanti Polar Lipids公司产品）在正癸烷-正丁醇（体积比9:1）混合溶剂中的溶液制备得到。两个缓冲液室均加入1 mL 1 M KCl、10 mM Tris缓冲液，pH值为7.4，实验温度维持在25 °C。将LPPO添加至膜的顺式（cis）一侧，浓度设置为5、2.5或1.25 µg/ml。采用Ag/AgCl电极测量膜电流，施加的固定膜电位为50 mV。电流信号经LCA-200-100GV放大器（Femto公司）放大后，通过KPCI-3108或KPCI-3102采集卡（Keithley公司）进行数字化。信号后续使用QuB软件进行处理（引用文献：Nicolai, C. & Sachs, F. Solving ion channel kinetics with the QuB software. *Biophys. Rev. Lett.* 8, 191–211 (2013)）。