Mitotyping of necropsy samples from Taï National Park, Côte d'Ivorie

To molecularly verify the species of duiker carcasses found in TNP, we used a conventional PCR targeting a 709 bp segment of the mitochondrial cytochrome c oxidase. Amplicons of the expected size were purified and sent for Sanger sequencing. All resulting sequences were compared against the core nucleotide database of NCBI using BLASTn. For duikers N230505, N190204 and N200122 the PCR amplified a NUMT which are included. Also available is a bam file of competitive maps of N230505 HTS data to a collection of duiker mitochondrial genomes for species ID.为对塔伊国家公园（TNP）内发现的麂羚尸体样本开展分子物种鉴定，我们采用常规聚合酶链式反应（conventional PCR，PCR）靶向扩增线粒体细胞色素c氧化酶（mitochondrial cytochrome c oxidase）的一段709碱基对（bp）片段。将符合预期片段大小的扩增产物纯化后，送至桑格测序（Sanger sequencing）平台完成测序。通过BLASTn工具，将所有获取的序列比对至美国国家生物技术信息中心（National Center for Biotechnology Information，NCBI）的核心核苷酸数据库。针对编号为N230505、N190204与N200122的麂羚样本，本次PCR扩增得到的实为核线粒体DNA片段（nuclear mitochondrial DNA segment，NUMT），本数据集已收录该类片段。此外，本数据集还提供一份BAM格式文件，该文件包含将N230505的高通量测序（High-Throughput Sequencing，HTS）数据比对至麂羚线粒体基因组集合以进行物种鉴定的比对图谱。