Cav3.2-/- Mouse #334 CA1 EEG recording

A Cav3.2-/- mouse (internal #334) was implanted with an intrahippocampal electrode for electrohippocampal CA1 EEG recordings. The differential electrode of the TA10ETA-F20 transmitter (Data Science International, DSI, USA, technical specifications: weight 3.9 g, volume 1.9 cc, input voltage range ± 2.5 mV, channel bandwidth (B) 1–200 Hz, nominal sampling rate (f) 1000 Hz (f = 5 B), temperature operating range 34–41 °C) was positioned at the following stereotaxic coordinates: (+)-lead, caudal − 2 mm, lateral of bregma 1.5 mm (right hemisphere), and dorsoventral (depth) 1.5 mm. The epidural reference electrode was positioned on the surface of the cerebellar cortex at the following stereotaxic coordinates: (−)-lead, bregma − 6 mm and lateral of bregma 1 mm (right<br> hemisphere). The deep tungsten electrodes (FHC, USA) are encapsuled with epoxylite with an<br> impedance of 50–100 kΩ (measured at 1000 Hz) and a shank diameter of 250 μm. For further details on the Cav3.2 mouse model, animal experimentation approval, anaesthesia, transmitter insertion, stereotaxic electrode implantation, postoperative pain management and recovery, please refer to Arshaad MI et al., 2021. EEG data were exported to NeuroScore 3.2.9306-1 (Data Sciences International, DSI, USA). As the NeuroScore file format does not allow free access to the data, we have exported the raw EEG data as asci.-files, so that they can easily be imported in secondary analysis software. Importantly, the exported EEG data represent raw EEG data. No filtering, no artefact detection and/or artefact removal have been carried out. Thus, it is the users responsibility to adequately pretreat the data before further processing and analysis. As described in Arshaad MI et al. (2021), filtering, artefact detection and removal was carried out using NeuroScore before further analysis. The EEG data are presented here as a zip-folder for size reasons. Four EEG recordings are exported: R1 (a first 24h long-term EEG recording 10 days post transmitter implantation), R2 (a second 24h long-term recording 17 days post transmitter implantation), U1 (a 6h EEG recording following the first urethane injection (800 mg/kg i.p.) at day 18 post transmitter implantation), U2 (a 6h EEG recording after a second urethane injection (800 mg/kg i.p.) at day 25 post transmitter implantation). In addition, relative activity values are exported for all four recordings. For details on the recording procedure, please refer to Arshaad MI et al. (2021).本研究选取内部编号为#334的Cav3.2基因敲除（Cav3.2-/-）小鼠，于其海马内植入电极，用于海马CA1区脑电图（Electroencephalogram, EEG）记录。 TA10ETA-F20发射器（Data Science International，DSI，美国）的差分电极技术参数如下：重量3.9 g，体积1.9 cc，输入电压范围±2.5 mV，通道带宽（B）1–200 Hz，标称采样率（f）1000 Hz（f = 5 B），工作温度范围34–41 ℃。该差分电极的立体定位坐标为：（+）引线：尾侧-2 mm，距前囟（bregma）外侧1.5 mm（右侧半球），背腹侧（深度）1.5 mm。 硬膜外参考电极置于小脑皮质表面，其立体定位坐标为：（-）引线：前囟处-6 mm，距前囟外侧1 mm（右侧半球）。 本研究使用的深部钨电极（FHC，美国）采用环氧树脂（epoxylite）封装，阻抗为50–100 kΩ（1000 Hz下测得），针杆直径250 μm。 关于Cav3.2小鼠模型、动物实验伦理审批、麻醉、发射器植入、立体定位电极植入、术后疼痛管理与恢复的详细信息，请参见Arshaad MI等人2021年的研究。 脑电图数据导出至NeuroScore 3.2.9306-1软件（Data Sciences International，DSI，美国）。由于NeuroScore的文件格式不支持直接开放访问原始数据，我们将原始脑电图数据导出为ASCII文件，以便用户可轻松将其导入至二次分析软件中。 需特别说明：本次导出的脑电图数据均为原始数据，未进行滤波、伪迹检测或伪迹移除操作。因此，用户需在后续处理与分析前对数据进行适当的预处理。如Arshaad MI等人（2021）所述，在开展进一步分析前，可通过NeuroScore完成滤波、伪迹检测与移除操作。 考虑到文件存储大小，本次提供的脑电图数据以ZIP压缩包形式打包。本次共导出4组脑电图记录：R1为发射器植入后第10天的首次24小时长期脑电图记录；R2为发射器植入后第17天的第二次24小时长期脑电图记录；U1为发射器植入后第18天首次腹腔内（intraperitoneal, i.p.）注射乌拉坦（800 mg/kg）后的6小时脑电图记录；U2为发射器植入后第25天第二次腹腔内（intraperitoneal, i.p.）注射乌拉坦（800 mg/kg）后的6小时脑电图记录。 此外，我们还为4组记录分别导出了相对活动度数值。关于记录流程的详细信息，请参见Arshaad MI等人（2021）的研究。