MNase-seq data in 4 modified strains of Saccharomyces cerevisiae, treated or not with 10-Phenanthroline

We showed that the introduction of a 81bp DNA segment in phase genes leads to the generation of fuzzier nucleosome arrays, but neither to a significant change in the placement of the +1 and –last nucleosomes, nor to a coordinate sliding of nucleosomes. This suggests that irrespectively of other nucleosomes, the +1 and –last nucleosomes are placed in well-defined regions marked by sequence-dependent intrinsic and extrinsic factors, while the rest of the nucleosomes are placed based on periodicity considerations.本研究证实，在相位基因（phase genes）中引入一段81碱基对（bp）的DNA片段，会生成定位更为模糊的核小体（nucleosome）阵列；但该操作既未使+1核小体与-末位核小体的定位发生显著变化，也未引发核小体的协同滑动。这表明，不受其他核小体的影响，+1与-末位核小体会被定位在由序列依赖性内源性与外源性因子所标记的明确区域中，而其余核小体的定位则基于周期性规则进行排布。