spiked labeled lymphocyte in unlabeled WBCs

To prove the ability of our microflow cytometer, experiments on sorting labeled lymphocyte from unlabeled WBCs are conducted. The collected cells from target and waste outlets were analysed using commercial BD flow cytometer. Experiments on fetal nucleated red blood cells (FNRBCs) isolation from maternal blood samples are also conducted.
Conclusion:
The recovery rate for spiked experiments is basically stable at 80%. For target cells ratio up to 1/600000, our µFACS device can achieve more than 98% WBC removal rate. For FNRBCs sorting experiments, sequencing results show that male cells can be found in some samples.