Transcriptome sequencing of ovarian granulosa cells

Polycystic ovary syndrome (PCOS) is one of the most prevalent endocrine disorders in women of reproductive age. However, the underlying molecular mechanism remains unclear. In this study, we employed RNA sequencing analysis to identify differentially expressed protein-coding genes and long non-coding RNA (lncRNA) expression profiles in granulosa cells from women with and without PCOS. A total of 10,159 lncRNA transcripts were identified in the PCOS and control groups, of which 32 were found to be differentially expressed. It was established that the level of NONHSAT233728.1 was diminished in women with PCOS. The present study demonstrated the role of NONHSAT233728.1 in granulosa cells from patients with PCOS and further investigated the potential mechanism of NONHSAT233728.1 in the KGN cell line.