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Data from: Population and individual identification of Coho Salmon in British Columbia through parentage-based tagging and genetic stock identification: an alternative to coded-wire tags

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DataONE2017-06-13 更新2024-06-26 收录
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Parentage-based tagging (PBT) and genetic stock identification (GSI) were used to identify individual Coho Salmon (Oncorhynchus kisutch) to specific populations and broodyears. In total, 20,242 individuals from 117 populations were genotyped at 304 single nucleotide polymorphisms (SNPs) via direct sequencing of amplicons. Coho Salmon from 15 populations were assigned via parentage analysis that required the genotypes of both parents. The overall accuracy of assignment for 1,939 Coho Salmon to the correct population was 100%, and to correct broodyear within population was also 100%. Inclusion of individuals requiring only a single parental genotype for identification resulted in assignments of 2,101 individuals, with an accuracy of 99.95% (2,000/2,001) to population and 100.0% to age. With 23 regions defined by the CWT program, and individuals displaying an assignment probability < 0.85 excluded from the analysis, average regional assignment accuracy of individuals via GSI was 98.4% over all 23 regions. A PBT/GSI or PBT system of identification will provide an alternate method of identification in the assessment and management of Canadian-origin Coho Salmon relative to the existing CWT program.

基于亲权的标记法(Parentage-based tagging, PBT)与遗传种群鉴定(genetic stock identification, GSI)被用于将单个银大麻哈鱼(Oncorhynchus kisutch)鉴定至特定种群与繁育年度。本数据集共对来自117个种群的20242个个体,通过扩增子直接测序技术完成了304个单核苷酸多态性(SNPs)位点的基因分型。其中15个种群的银大麻哈鱼通过需双亲基因型参与的亲权分析完成个体归属判定;针对1939尾银大麻哈鱼的种群归属总体准确率达100%,种群内繁育年度归属准确率同样为100%。纳入仅需单亲本基因型即可完成鉴定的个体后,共完成2101个个体的归属判定,其种群归属准确率为99.95%(2000/2001),年龄归属准确率为100.0%。以CWT程序划定的23个区域为分析单元,剔除归属概率低于0.85的个体后,基于GSI的个体区域归属平均准确率在全部23个区域中达98.4%。相较于现有CWT程序,基于PBT/GSI或PBT的鉴定体系可为加拿大原产银大麻哈鱼的资源评估与管理提供一种替代鉴定方法。
创建时间:
2017-06-13
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