Baseline Expression of Inflammatory Cytokines and Receptors in the Normal Human Breast

Immortalized human breast cancer cell line, Normal, was analyzed via RT-qPCR for transcript expression of selected cytokines and cytokine receptors associated with promotion of tumor vasculature and breast cancer metastasis An array of inflammatory cytokines and matching receptors that have been associated with the promotion of tumor vasculature and breast cancer metastasis were identified based on literature and clinical data. Using this array, we screened normal breast tissue, procured from the Simmons Cancer Instititute Tissue Bank at Southern Illinois University School of Medicine, along with seven breast cancer cell lines (T47D, MCF7, ZR-75, HCC1806, MDA-MB-231, HCC1954, SUM149), representing a range of breast cancer types. These results can be analyzed individually or used to compare the expression differences in normal breast tissue to breast cancer cell lines to identify potential mediators of autocrine or paracrine promotion of tumor angiogenesis, lymphangiogenesis and metastasis. Each cell line is published separately in GEO.