Supplementary material 3 from: Machado A, Rodríguez-Expósito E, López M, Hernández M (2017) Phylogenetic analysis of the genus Laparocerus, with comments on colonisation and diversification in Macaronesia (Coleoptera, Curculionidae, Entiminae). ZooKeys 651: 1-77. https://doi.org/10.3897/zookeys.651.10097

Chronogram of genus Laparocerus Schönherr, 1834 from Macaronesia (Coleoptera, Curculionidae, Entiminae)
: Explanation note: Timetree generated with MEGA7 (Kumar et al. 2016) using the RelTime method. Divergence times for all branching points in the Bayesian phylogram (COII, 16S rRNA, 12S rRNA) calculated using Maximum Likelihood method with the General Time Reversible model. Relative times were optimized and converted to absolute divergence times (shown next to branching points) based on not allowing species cluster marked with ♦ to be older than the age of the island of La Palma (1,72 Ma) or younger than 0.21 Ma. Bars around each node represent 95% confidence intervals which were computed using the method described in Tamura et al. (2013). The estimated log likelihood value is -32807.3327. A discrete gamma distribution was used to model evolutionary rate differences among sites (6 categories (+G, parameter = 0.5622)). The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 42.7318% sites). The analysis involved 255 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 1357 positions in the final dataset. Taxon codes include an abbreviation of species name, its code number, and the initial of the island of origin (see Appendix 1).