Quantitative DIA-based proteomic analysis of lysosomes (Lyso-IP) from VPS35[D620N] Mouse Embryonic Fibroblasts (MEFs)

This Zenodo deposit contains a publicly available description of the Dataset: Title: "Quantitative DIA-based proteomic analysis of lysosomes (Lyso-IP) from VPS35[D620N] Mouse Embryonic Fibroblasts (MEFs)". Description: Quantitative DIA-based proteomic analysis comparing isolated lysosomes (Lyso-IP) from Wild-Type and VPS35[D620N] knock-in MEFs. This dataset provides the basal characterization of mutant lysosomal protein changes without inhibitor treatment. Expression of the 3XHA-TMEM192 tag was verified by genotyping PCR and anti-HA Western Blotting. Data acquired on an Orbitrap Exploris 480 and processed using DIA-NN 1.8.1. This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual. This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF). This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset本Zenodo存档包含该数据集的公开说明： 标题："基于数据非依赖采集（Data-Independent Acquisition, DIA）的定量蛋白质组学分析：VPS35[D620N]突变小鼠胚胎成纤维细胞（Mouse Embryonic Fibroblasts, MEFs）的溶酶体免疫沉淀（Lyso-IP）产物" 描述：本数据集为基于DIA的定量蛋白质组学研究，对比野生型与VPS35[D620N]敲入型小鼠胚胎成纤维细胞的分离溶酶体免疫沉淀产物。该数据集表征了未施加抑制剂处理的突变型溶酶体蛋白质组的基础变化特征。研究人员通过基因分型PCR与抗HA免疫印迹（Western Blotting）验证了3XHA-TMEM192标签的表达情况。所有数据采集于Orbitrap Exploris 480质谱仪，并使用DIA-NN 1.8.1软件进行处理。 本数据集可通过ASAP CRN云平台（cloud.parkinsonsroadmap.org）向研究人员开放获取。访问申请的具体流程详见用户手册。 本研究由帕金森病研究对齐科学协作网络（Aligning Science Across Parkinson's Collaborative Research Network, ASAP CRN）通过迈克尔·J·福克斯帕金森病研究基金会（Michael J. Fox Foundation for Parkinson's Research, MJFF）资助。 本Zenodo存档由ASAP CRN云平台工作人员代表数据集作者创建，可为CRN云平台数据集提供可引用的参考文献。