Disinfectant efficacy on mixed biofilms comprising Escherichia coli and spoilage microorganisms

This study aimed to investigate the impact of temperature and the presence of other microorganisms on the susceptibility of STEC to biocides. Mature biofilms were formed at both 10°C and 25°C. An inoculum of planktonic bacteria comprising 106 CFU/ml of spoilage bacteria and 103 CFU/ml of a single E. coli strain (O157, O111, O103, and O12) was used to form mixed biofilms. The following bacterial combinations were tested: T1: Carnobacterium piscicola + Lactobacillus bulgaricus +STEC, T2: Comamonas koreensis + Raoultella terrigena + STEC, and T3: Pseudomonas aeruginosa + C. koreensis + STEC. Tested biocides included quaternary ammonium compounds (Quats), sodium hypochlorite (Shypo), sodium hydroxide (SHyd), hydrogen peroxide (HyP), and BioDestroy®-organic peroxyacetic acid (PAA). Biocides were applied to 6-day-old biofilms. Minimum Bactericidal Concentrations (MBC) and Biofilm Eradication Concentrations (BEC) were determined. Planktonic cells and single-species biofilms exhibited greater s..., The dataset was collected through a series of experiments designed to evaluate the efficacy of various disinfectants against different bacterial strains, including Shiga toxin-producing Escherichia coli (STEC), spoilage bacteria (SP), and lactic acid bacteria (LAB).
A total of nine STEC, four spoilage bacteria (SP), and six lactic acid bacteria (LAB) strains were included in this study. 
Six disinfectants commonly employed in the food industry were evaluated. Disinfectants were prepared according to the manufacturer's specified concentrations. Stock solutions were prepared in sterile hot water (50°C) and maintained at 40° to 50°C. Solutions were used within 30 min after preparation. Before initiating testing, the active chlorine concentration in the sodium hypochlorite solution was tested using a chlorine analysis method (Total) Test Kit (HACH, Model CN-65, Ontario, Canada).
STEC strains [E. coli O145 (75-83), E. coli O157:H7 (1934), E. coli O103:H2 (99-2076), and E. coli O157:H7 (R508)..., , # Disinfectant efficacy on mixed biofilms comprising *Escherichia coli* and spoilage microorganisms

[https://doi.org/10.5061/dryad.zpc866tgh](https://doi.org/10.5061/dryad.zpc866tgh)

## Description of the data and file structure

The provided data files are mostly optical density data collected from 96-well microplates containing STEC biofilms. Another set of data also contains an enumeration of the bacterial strains used in this study within multispecies and single-species biofilms.

The data used in the study was gathered in Canada in a Biosafety level 2 laboratory (BSL2) from 2021 to 2023. This study aimed to determine Shiga toxigenic *E. coli* (STEC) strains' capacity to form multispecies biofilms and to test the biofilm's susceptibility to different biocide.

### Data variables:

* Label: unique identifier
* REP: replicate number
* Sanitizer: Tested biocides included quaternary ammonium compounds (Quats), sodium hypochlorite (Shypo), sodium hydroxide (SHyd), hydrogen peroxi...

本研究旨在探究温度与其他微生物的存在对产志贺毒素大肠杆菌（Shiga toxin-producing Escherichia coli, STEC）的杀菌剂敏感性的影响。分别于10℃与25℃下构建成熟生物被膜。采用包含10^6 CFU/ml腐败菌（Spoilage Bacteria, SP）与10^3 CFU/ml单一大肠杆菌菌株（O157、O111、O103及O12）的浮游细菌接种物，以制备混合生物被膜。测试的细菌组合如下：T1：嗜肉杆菌（Carnobacterium piscicola）+保加利亚乳杆菌（Lactobacillus bulgaricus）+STEC；T2：韩国丛毛单胞菌（Comamonas koreensis）+特氏劳特罗菌（Raoultella terrigena）+STEC；T3：铜绿假单胞菌（Pseudomonas aeruginosa）+韩国丛毛单胞菌（Comamonas koreensis）+STEC。测试用杀菌剂包括季铵盐类化合物（Quats）、次氯酸钠（Shypo）、氢氧化钠（SHyd）、过氧化氢（HyP）以及BioDestroy®有机过氧乙酸（PAA）。将杀菌剂施加于培养6天的生物被膜，测定最小杀菌浓度（Minimum Bactericidal Concentrations, MBC）与生物被膜根除浓度（Biofilm Eradication Concentrations, BEC）。浮游菌与单菌种生物被膜表现出更强的[原文截断]。

本数据集通过一系列旨在评估各类消毒剂对不同细菌菌株消杀效果的实验收集所得，受试菌株包括产志贺毒素大肠杆菌（STEC）、腐败菌（SP）与乳酸菌（Lactic Acid Bacteria, LAB）。
本研究共纳入9株STEC、4株腐败菌（SP）及6株乳酸菌（LAB）。
本研究评估了食品工业中常用的6种消毒剂。消毒剂按照生产商指定浓度配制：母液以无菌热水（50℃）配制，并维持于40℃至50℃之间，配制后30分钟内完成使用。试验启动前，采用总氯分析检测试剂盒（HACH，型号CN-65，加拿大安大略省）测定次氯酸钠溶液中的有效氯浓度。
STEC菌株[大肠杆菌O145 (75-83)、大肠杆菌O157:H7 (1934)、大肠杆菌O103:H2 (99-2076)及大肠杆菌O157:H7 (R508)……]

# 大肠杆菌与腐败微生物构成的混合生物被膜的消杀效果

[https://doi.org/10.5061/dryad.zpc866tgh](https://doi.org/10.5061/dryad.zpc866tgh)

## 数据与文件结构说明

本研究提供的数据文件多为取自包含STEC生物被膜的96孔微孔板的光密度数据。另一组数据则包含多菌种与单菌种生物被膜中所用细菌菌株的计数结果。
本研究数据于2021年至2023年间在加拿大的二级生物安全实验室（Biosafety Level 2, BSL-2）中采集。本研究旨在明确产志贺毒素大肠杆菌（STEC）菌株形成多菌种生物被膜的能力，并测试该生物被膜对各类杀菌剂的敏感性。

### 数据变量：
* 标签（Label）：唯一标识符
* 重复次数（REP）：重复实验编号
* 消毒剂（Sanitizer）：测试用杀菌剂包括季铵盐类化合物（Quats）、次氯酸钠（Shypo）、氢氧化钠（SHyd）、过氧化氢[原文截断]