Microglia control the formation of brain small vessel calcification via TREM2

The role of microglia in the formation of brain capillary calcification was investigated in a platelet-derived-growth factor B (PDGFB) hypomorph - Pdgfb ret/ret mouse model of human primary familial brain calcification. RNA-seq was performed on brain tissue developing vessel-associated calcifications (n=4 thalamus) and on brain tissue lacking calcifications (n=4 cortex) of Pdgfb ret/ret animals. Control animals (Pdgfb wt/ret) do not develop calcifications in any of the analyzed regions (n=4 thalamus and n=4 cortex).本研究以模拟人类原发性家族性脑钙化的血小板衍生生长因子B（platelet-derived-growth factor B，PDGFB）低功能亚型Pdgfb^ret/ret小鼠模型为对象，探究小胶质细胞（microglia）在脑毛细血管钙化形成过程中的作用。研究对Pdgfb^ret/ret小鼠中存在血管相关钙化的脑组织（丘脑样本，n=4）以及无钙化的脑组织（皮层样本，n=4）进行了RNA测序（RNA-seq）。对照组小鼠（Pdgfb^wt/ret）在所有检测区域均未出现钙化（丘脑样本n=4，皮层样本n=4）。