Thermodynamic competition and methane oxidation differentially regulate CH4 flux across hydrologically and geochemically diverse wetlands

This is the data corresponding to the paper studying methane dynamics in 6 wetlands in North Dakota from the Prairie Pothole Region (PPR) of North America. Data used in this study includes newly collected microbiology data (16S rRNA amplicon sequencing, metagenomics, metatranscriptomics), and enviornmental geochemistry (methane and carbon dioxide flux and porewater concentration and methane isotope data). The newly assembled metagenome assembled genomes (MAGs) were combined with MAGs from the MUCC v2.0.0 (10.5281/zenodo.14532347) database to create a larger ecosystem database that the metatranscriptomes were mapped to.本数据集配套于一项研究论文，该论文针对北美草原坑洼区（Prairie Pothole Region，PPR）北达科他州境内的6处湿地开展甲烷动态相关研究。本研究使用的数据包含新采集的微生物组学数据（16S rRNA扩增子测序、宏基因组学、宏转录组学）以及环境地球化学数据，具体包括甲烷与二氧化碳通量、孔隙水浓度及甲烷同位素数据。新组装的宏基因组组装基因组（Metagenome Assembled Genomes，MAGs）与MUCC v2.0.0数据库（DOI: 10.5281/zenodo.14532347）中的MAGs进行整合，构建了更大规模的生态系统数据库，用于宏转录组数据的比对映射。