Effect of clinically observed BTK mutations on gene expression during cell proliferation and differentiation in TMD8 cells

To investagate the clinically observed BTK C481S, C481F, C481Y and C481R mutations in the regulation of B cell receptor signaling, we extablished TMD8 cells expressing BTK C481S, C481F, C481Y and C481R in which endogenous BTK was inactivated by ibrutinib. We then performed gene expression profiling analysis using data obtained from RNA-seq of 20 different cells after DMSO or 10 nM ibrutinib treatment from two indenpendent experiments. Comparative gene expression profiling analysis of RNA-seq data from parental TMD8 cells and TMD8 cells expressing BTK C481S, C481F, C481Y and C481R after DMSO or 10 nM ibrutinib treatment 24 hours.