Snorkel-tag Based Affinity Chromatography for Recombinant Extracellular Vesicle Purification

Getting insights on tissue or cell specific EV composition in pathophysiological conditions for developing therapeutics, biomarkers and diagnostic tools in modern medicine still remains a major bottleneck. Similarly, lack of standards for EV comparison also hampers progression in the field. Here, we therefore generated a tagged CD81 fusion protein highly enriched in EVs, enabling affinity isolation of EVs from complex matrices in a non-destructive and pure form without disturbing the here tested EV characteristics. Conditioned media from WT and CD81-snorkel tag overexpressing WJ-MSCs were concentrated by ultrafiltration. CD81-snorkel tag EVs from concentared conditioned media were purified by StEVAC method.