Proteomics of Extracellular Vesicles in mild and severe dengue patients’ plasma

We have isolated extracellular vesicles from healthy donors' plasma, other febrile illness plasma, mild dengue patients (Platelet counts= >100,000 per mm3 of blood) , and severe dengue patients' plasma (Platelet counts= <30,000 per mm3 of blood) by ultracentrifugation. For understanding, the differences in protein cargo among the EVs isolated from these groups were subjected to SWATH analysis. We have used three biological replicates of each group of EVs. A- EVS DERIVED FROM SEVERE DENGUE PLASMA B- EVS DERIVED FROM MILD DENGUE PLASMA C- EVS DERIVED FROM OTHER FEBRILE ILLNESS PLASMA D- EVS DERIVED FROM HEALTHY DONORS PLASMA