Effect of Arbuscular Mycorrhizal Fungi on Assembly of Rhizospheric and Endophytic Bacterial Community in Tomato

In recent years, facility agriculture has developed rapidly in China, but chemical fertilizers have been still used to ensure production, so it is important to develop green ecological fertilizers. Arbuscular mycorrhizal (AM) fungi are a kind of ubiquitous symbiotic fungi on the earth, which can improve stress tolerance and promote growth for plant, and have great application potential. However, few studies have revealed the effects of AM fungi on recruitment of bacteria in roots of tomato grown in facility. A greenhouse pot experiment was used to study the effects of inoculation with Funneliformis mosseae (FM) and Rhizophagus intraradices (RI) on assembly of rhizospheric and endophytic bacterial community in tomatoes. The results showed that both FM and RI increased the Sobs and Ace index in rhizospheric bacterial communities; compared with non-inoculated. FM decreased the Ace index, and RI decreased the Sobs index in endophytic bacterial community. RI increased the Simpson index in rhizospheric and endophytic bacterial community, reducing diversity. Both FM and RI significantly increased the relative abundance of the Chloroflexi phylum, compared with non-inoculated, and RI treatment significantly increased the relative abundance of Arthrobacter genus. Network structure analysis revealed that both AM fungi all increased the positive relationship between the bacteria of roots. Lactobacillus was a keystone taxa of endophytic bacterial community in FM treatment and Streptomyces was that in RI treatment. These results suggest that both AM fungi can affect the assembly of the bacterial community of roots in facility tomatoes, and inclined to enrich the bacteria involved in nitration reaction or cooperation relationships. It provides theoretical support for the application of AM fungi in facility cultivation of tomatoes.