Transcriptome analysis using Quant-RNAseq after NPM1 or HIF-1a silencing under normoxia or Hypoxia

Hypoxia inducible factor-1 (HIF-1) is a central transcriptional regulator of genes associated with adaptive responses to hypoxia. NPM1 is a histone chaperone found to associate with HIF-1a in a phosphorylation dependent manner and increase its activty. The aim of this study was to find if HIF-1a and NPM1 regualate gene expression under hypoxia. Transcriptome analysis using Quant-RNA-seq after HIF-1a or NPM1 silencing under hypoxia reveals a significant number of genes, the hypoxic expression of which depends on both proteins. Overall design: Examinaton of genes expressed after silencing with siRNA NPM1 or HIF-1a in HeLa cells and subsequent incubaton in normaxia or hypoxia.