Proteomics raw data of mammary tumor cells from female dogs

This data is part of the material used to study the effect of rapamycin on mammary tumor cells of female dogs. We would like to evaluate the dose response of mammary tumor cells from dogs and evaluate the proteins comparing before and after treatment with rapamycin. We used samples of tumors from mammary gland and their respective metastases from 2 animals. Four samples were cultured in vitro and treated with rapamycin in different doses (IC50) for each cell line. The cells were prepared to proteomics (ESI Q-TOF MS/MS). We used for statistical analysis comparisons between control versus rapamycin treatment groups, and also metastasis versus primary tumor versus metastasis rapamycin versus primary tumor rapamycin. The experiment was performed in biological duplicates and in technical duplicates.

本数据集为研究雷帕霉素（rapamycin）对雌性犬乳腺肿瘤细胞作用的实验材料的一部分。本研究旨在评估犬源乳腺肿瘤细胞的剂量响应效应，并对比经雷帕霉素处理前后的蛋白质表达差异。我们采集了2只受试犬的乳腺肿瘤组织及其对应的转移灶样本，选取其中4份样本开展体外培养，并针对每株细胞系施加不同剂量（半数抑制浓度IC50）的雷帕霉素进行干预。随后完成了用于蛋白质组学（ESI Q-TOF MS/MS）检测的细胞样本制备工作。本次统计分析涵盖三组对照比较：对照组与雷帕霉素处理组的对比、原发肿瘤与转移灶的对比，以及经雷帕霉素处理的转移灶与经雷帕霉素处理的原发肿瘤的对比。本实验设置了生物学重复与技术重复各两次。