Effects of methotrexate treatment on hepatic metabolism in obesity. Mus musculus

Analysis of metabolic changes in liver from obese mice fed on 60% high fat diet and treated with chemotherapeutic agent methotrexate (50 mg/kg) with a minimum of . Overall design: One color experiment. Our conditions: 1) Low fat diet, No treatment, 2) Low fat diet, Methotrexate 50 mg/kg treatment, 3) High fat diet, No treatment, 4) High fat diet, Methotrexate 50mg/kg treatment. A minimum of 3 replicates (3-5) for each condition.