Dataset to the article "Development of PTSD-like symptoms in susceptible individuals correspond to alterations in gut microbiome and integrity in a rat model"

This dataset was generated for a manuscript currently under peer review: This dataset was generated for a manuscript currently under peer review: Authors: Csilla Lea Fazekas1,2,3$, Krisztina Bánrévi1$, Pedro Correia1,2,3, Bibiána Török1,2,3, Adrienn Szabó1,2,3, Peter Karailiev4, Manon Bellardie1, Eszter Sipos1, Ferenczi Szilamér1, Anita Bufa5, Kitti Mintál2, Zoltán S. Zádori6, Daniela Jezova4$ and Dóra Zelena1,2, $* Title: Development of PTSD-like symptoms in susceptible individuals correspond to alterations in gut microbiome and integrity in a rat model Submitted to Microbiome The dataset corresponds to the experiments and analyses described in the manuscript and will be updated with the article DOI upon publication. Description:This dataset contains experimental and analytical data generated in a rat model of post-traumatic stress disorder (PTSD) based on footshock trauma. Male Long Evans rats (14–18 weeks old) were divided into control (non-shocked), resilient, and vulnerable groups based on behavioral z-scores derived from anxiety, fear, and sociability tests. Behavioral classification and grouping details are described in a previously published study (doi: 10.1080/10253890.2020.1795121). Data types included: Behavioral data z-score classification data for group assignment Physiological and tissue data Small intestine length Histology data (ileum) Quantitative measurements of villi height and thickness Microbiome data Processed taxonomic tables Metabolomics data Short-chain fatty acid (SCFA) concentrations measured from feces and plasma Gene expression data (qPCR) mRNA levels for ileum and PFC Genes measured include: Muc2, Reg3β, CAMP, DEF5α, TLR-4, TNFα, IL-1β, IL-10, ZO-1, Occludin, Claudin-1/8/11/12. Primers listed in Table 1 of the manuscript Metadata and processing notes: Raw data are organized into an Excel file, each row containg all the data from a single animasl, while column heading containing teh type of measurement in the given column. Statistical analysis was performed using StatSoft STATISTICA 13.4; Levene’s test, one-way ANOVA, repeated measures ANOVA, Fisher LSD post hoc tests, and Pearson correlations were used as appropriate. The mean ±SEM values are in Supplementary Tables as well as represented on Figures. Access restricted for peer review purposes. Data available to reviewers via private link.本数据集为一篇目前处于同行评审阶段的学术手稿所生成。 作者：Csilla Lea Fazekas¹,²,³$, Krisztina Bánrévi¹$, Pedro Correia¹,²,³, Bibiána Török¹,²,³, Adrienn Szabó¹,²,³, Peter Karailiev⁴, Manon Bellardie¹, Eszter Sipos¹, Ferenczi Szilamér¹, Anita Bufa⁵, Kitti Mintál², Zoltán S. Zádori⁶, Daniela Jezova⁴$ 与 Dóra Zelena¹,²,*（注：$代表共同第一作者，*代表通讯作者） 研究标题：大鼠模型中易感个体创伤后应激障碍（Post-traumatic Stress Disorder, PTSD）样症状的发生与肠道菌群及肠道完整性改变相关 已投稿至《Microbiome》期刊 本数据集对应手稿中描述的全部实验与分析内容，文章正式发表后将补充该文章的数字对象标识符（DOI）。 数据集说明：本数据集包含基于足底电击造模的创伤后应激障碍（PTSD）大鼠模型所产生的实验与分析数据。研究选取14~18周龄的雄性Long Evans大鼠，基于焦虑、恐惧与社交行为测试得到的行为学Z分数，将其分为对照组（未接受足底电击）、应激抵抗组与应激易感组。行为学分类与分组的详细方法已在既往发表的研究中阐明（DOI: 10.1080/10253890.2020.1795121）。 包含的数据类型： 1. 行为学数据：用于分组的Z分数分类数据 2. 生理学与组织学数据： - 小肠长度 - 回肠组织学数据 - 绒毛高度与厚度的定量测量结果 3. 菌群组学数据： - 已处理的分类学数据表 4. 代谢组学数据： - 粪便与血浆中短链脂肪酸（Short-Chain Fatty Acid, SCFA）浓度检测结果 5. 基因表达数据（定量聚合酶链反应，quantitative polymerase chain reaction, qPCR）： - 回肠与前额叶皮层（Prefrontal Cortex, PFC）的mRNA表达水平 - 检测基因包括：Muc2、Reg3β、CAMP、DEF5α、TLR-4、TNFα、IL-1β、IL-10、ZO-1、Occludin、Claudin-1/8/11/12 - 引物序列详见手稿中的表1 元数据与处理说明： 原始数据整理于Excel文件中，每一行对应单只实验动物的全部检测数据，列标题则标注对应列的检测指标类型。 统计分析采用StatSoft STATISTICA 13.4软件完成；根据分析需求选用了莱文检验（Levene’s test）、单因素方差分析（one-way ANOVA）、重复测量方差分析（repeated measures ANOVA）、Fisher LSD事后检验以及Pearson相关分析。均值±标准误（standard error of the mean, SEM）结果可见于补充表格与各图中。 本数据集仅用于同行评审工作，评审专家可通过私有链接获取数据。