Obesity synergizes with HBx, Src and p53 mutation accelerate hepatocarcinogenesis [Microarray]

Previous studies have identified liver cancer associated to NASH, diabetes, obesity, and some genetic risk factors. We want to investigate the synergism between diet and genetic risk factors in hepatocarcinogenesis using omics data of four background zebrafish in three types of diet. We found the fishes have more genetic risk factors at the same time, and have higher probability to accelerate cancer formation. Overfed and high fat diet will increase the chance. Moreover, the results showed metabolism and genetic information processing, including the pathways of fatty acid metabolism, steroid biosynthesis and ribosome biogenesis are highly affected in hepatocellular carcinoma. Four background zebrafish in three types of diet: Wild type, HBx(p53-), Src(p53-), and HBx,Src(p53-) zebrafish in normal, overfed, and high fat diet. RNA was prepared from liver and analyzed by microarray.