GenBank accession numbers for ammonia oxidizer genes collected on the R/V Endeavor (SQO-Delta) in the San Francisco Bay Delta during September and October 2007.
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<p>Surface sediment samples were collected for potential nitrification rates, using sediment slurries with filtered site water. Ammonia oxidizer gene abundances (AOA and AOB <em>amoA</em>) were quantified using qPCR, and clone libraries for each gene were sequenced using Sanger sequencing.</p>
<p><strong>Related Manuscript: Damashel <em>et al., </em>2015</strong></p>
<p> </p>
本研究采集表层沉积物样品，采用过滤后的原位水配制沉积物悬液以测定潜在硝化速率。通过实时荧光定量PCR（quantitative PCR, qPCR）定量分析氨氧化菌功能基因——氨氧化古菌（Ammonia-Oxidizing Archaea, AOA）与氨氧化细菌（Ammonia-Oxidizing Bacteria, AOB）的amoA基因——的丰度，并利用桑格测序（Sanger sequencing）完成各基因克隆文库的测序工作。
相关研究文献：Damashel 等，2015
创建时间：
2025-03-09
