The whole transcriptome expression profiling of human macrophages; comparison between control and human milk exosomes capsulated oligosaccharides treatment

To discover the regulatory role of human milk exosomes oligosaccharides, The full cDNA was extracted from human macrophages and then compared the expression profiles of them to discovery the key features regulated by human milk exosomes extracted oligosaccharides. There are two groups cells used for this expression profile comparison: control and human milk exosomes extracted oligosaccharides treated. The RNA was extracted from well cultured cells using TRIzol reagent, and whole transcriptome gene expression profiling were performed using an Affymetrix Human Gene 1.1 ST whole transcriptome microarray platform.