Data_origin_Xenopus_laevis_Europe

This dataset contains data and supplementary information from the manuscript:'Unequal contribution of native South African phylogeographic lineages to the invasion of the African clawed frog, Xenopus laevis, in Europe' authors:C. De Busschere, J. Courant, A. Herrel, R. Rebelo, D. Rödder, G.J.Measey, T. Backeljau contact:[e-mail] Short description files: 16S.nexus Nexus file generated by DnaSP Ver. 5.10.01 containing all the 16S sequences used in the manuscript. See file 'Online resource 1' for Genbank accesssion numbers and full sample information. cytb.fas Fasta file containing all the cytb sequences used in the manuscript. See file 'Online resource 1' for Genbank accesssion numbers and full sample information. prmt6.nex Nexus file generated by DnaSP Ver. 5.10.01 containing all the phased prmt6 sequences used in the manuscript. Haplotype phases were reconstructed from the raw data using the algorithm PHASE 2.1 implemented in DNAsp Ver. 5.10.01. See file 'Online resource 1' for Genbank accesssion numbers and full sample information. Mastl.nex Nexus file generated by DnaSP Ver. 5.10.01 containing all the phased Mastl sequences used in the manuscript. Haplotype phases were reconstructed from the raw data using the algorithm PHASE 2.1 implemented in DNAsp Ver. 5.10.01. See file 'Online resource 1' for Genbank accesssion numbers and full sample information. AR.nex Nexus file generated by DnaSP Ver. 5.10.01 containing all the phased AR sequences used in the manuscript. Haplotype phases were reconstructed from the raw data using the algorithm PHASE 2.1 implemented in DNAsp Ver. 5.10.01. See file 'Online resource 1' for Genbank accesssion numbers and full sample information. Online resource 1 Table. 1 Overview of sample, locality and allelic information. GenBank accession numbers listed in bold correspond to sequences of previous studies. Online resource 2-5 Fig. MP and BI tree of unique concatenated Cytb-16S haplotypes Table. PCR cycle conditions Table. Pairwise Population Fst values based on pairwise nucleotide differences for 16S. Table. Pairwise Population Fst values based on nuclear allele frequencies.

本数据集包含来自以下学术手稿的数据及补充信息：《南非本土系统地理谱系对非洲爪蟾（Xenopus laevis）在欧洲入侵的不均等贡献》，作者为C. De Busschere、J. Courant、A. Herrel、R. Rebelo、D. Rödder、G.J. Measey、T. Backeljau，联系邮箱：[e-mail]。

各文件简述如下：
1. 16S.nexus：由DnaSP 5.10.01版本生成的Nexus格式文件，包含本手稿中使用的全部16S序列。有关GenBank登录号及完整样本信息，请参阅"在线资源1"。
2. cytb.fas：FASTA格式文件，包含本手稿中使用的全部细胞色素b（cytochrome b, cytb）序列。有关GenBank登录号及完整样本信息，请参阅"在线资源1"。
3. prmt6.nex：由DnaSP 5.10.01版本生成的Nexus格式文件，包含本手稿中使用的全部已分型prmt6序列。单倍型分型通过集成于DNAsp 5.10.01中的PHASE 2.1算法从原始测序数据中重构得到。有关GenBank登录号及完整样本信息，请参阅"在线资源1"。
4. Mastl.nex：由DnaSP 5.10.01版本生成的Nexus格式文件，包含本手稿中使用的全部已分型Mastl序列。单倍型分型通过集成于DNAsp 5.10.01中的PHASE 2.1算法从原始测序数据中重构得到。有关GenBank登录号及完整样本信息，请参阅"在线资源1"。
5. AR.nex：由DnaSP 5.10.01版本生成的Nexus格式文件，包含本手稿中使用的全部已分型AR序列。单倍型分型通过集成于DNAsp 5.10.01中的PHASE 2.1算法从原始测序数据中重构得到。有关GenBank登录号及完整样本信息，请参阅"在线资源1"。

配套在线资源说明：
- 在线资源1：表1 样本、采样地点及等位基因信息概览。其中以加粗字体标注的GenBank登录号对应既往研究中的序列。
- 在线资源2至5：图：唯一串联Cytb-16S单倍型的最大简约法（Maximum Parsimony, MP）与贝叶斯推断（Bayesian Inference, BI）系统发育树；表：聚合酶链式反应（Polymerase Chain Reaction, PCR）循环反应条件；表：基于16S核苷酸差异的群体间成对固定指数（Fixation index, Fst）值；表：基于核等位基因频率的群体间成对固定指数（Fixation index, Fst）值。