Whole-genome Nanopore sequencing for native mtDNA methylation analysis
收藏NIAID Data Ecosystem2026-03-12 收录
下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP333847
下载链接
链接失效反馈官方服务：
资源简介：
We aim to establish and validate a workflow to detect mitochondrial DNA methylation from blood- and iPSC neuron-derived DNA.Therefore blood-derived DNA from five patients with Parkinson's disease and three control subjects was analyzed. As well as DNA from iPSC-derived midbrain neurons from four patients and three controls.To validate the pipeline, fully methylated and unmethylated synthetic DNA was analyzed at different dilution ratios (0%, 50%, 100%). Further, to get a baseline for false-positive methylation calls, two overlapping long-range PCR amplicons of the mtDNA were analyzed as well.
创建时间：
2021-09-18
