Mass spectrometry-based proteomic approach to colorectal cancer identified the cancer-associated proteins whose expression changes with the disease progression

Tumor and non-tumor tissues from 19 cases of colorectal cancer were examined using DIA-based mass spectrometry. A total of 11,833 proteins were identified, including 531 of the 748 cancer-associated proteins defined in the Catalogue of Somatic Mutations in Cancer. In the comparative analysis between the tumor and non-tumor tissue groups, we observed a significant number of differentially expressed proteins, some of which consistently increased or decreased with disease progression.