Complete response rates to combination therapy with sequential RFA and recombinant vaccine.

MC38-CEA+ cells were transplanted on day 0 on the right flank of CEA-Tg mice (n = 25–29/group). MC38-CEA+ tumors received sham or low-dose RFA (10–30 s at 70°C) on day 12, followed by high-dose ablative RFA (30–300 s at 70°C) on day 15. Vaccinated mice received rMVA-CEA/TRICOM s.c. on day 4 and rF-CEA/TRICOM on day 11 and every 7 days thereafter, alone or in combination with sequential RFA. Results are depicted as percentage of mice with complete responses or durable complete responses following treatment with RFA sham, sequential RFA, vaccine combined with RFA sham, or vaccine with sequential RFA. Values in parentheses depict number of responders/number of eligible animals in each cohort. P values denote statistical analysis of response rates to sequential RFA alone or in combination with vaccine, as determined by contingency analysis (Fisher’s exact test).