Quantitative analysis of transcriptomes in control and mTORC2-suppressed glioblastoma cells

We herein demonstrate that mammalian target of rapamycin complex 2 (mTORC2), a critical core component of the growth factor signaling system, globally alters histone modification as well as transcriptome through metabolic reprogramming in the highly malignant brain tumor glioblastoma (GBM). Integrated analyses unravel that mTORC2 regulates mineral metabolism including iron trafficking via histone H3K9 acetylation of the ferritin promoter, facilitating GBM growth and survival. Overall design: mRNA profiles of glioblastoma cell lines with Scramble or Rictor (a core component of mTORC2) knockdown were generated by deep sequencing.