Phenotype of donor Tregs before (pre) and after (post) G-CSF mobilization.

PBMCs were isolated before (pre, n = 25) and after (post, n = 21) G-CSF induced stem cell mobilization for FACS analyses. CXCR4 and CCR4 expression was determined on PBMCs isolated from n = 6 before and n = 7 SC donors after G-CSF induced SC mobilization. Expression values were calculated as percentage of CD4+CD25highCD127−Foxp3+ Tregs and are presented as mean values with standard deviation (SD). p values were calculated with two-tailed Student’s ttest.