OMIP-037: 16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets

The panel was developed to determine the combinational inhibitory receptor expression (“IR signatures”) of CD4+ T cells, CD8+ T cells, Natural Killer (NK) cells, invariant Natural Killer T (iNKT) cells, and gamma delta (γδ) T cells from individual human samples. The inhibitory receptors measured are PD-1, TIM-3, CD160, LAG-3, and TIGIT. The activation marker CD137 (4-1BB) is also included in the panel, as is IL-7Rα (CD127). This panel works well with cryopreserved PBMC from healthy and HIV-infected individuals well as fresh tumor specimens. For optimum performance of this panel with digested tumor specimens, the pan-lymphocyte marker CD45 is included (swapped for LAG-3). Other tissues/sample types have not yet been evaluated. Also, a modification of this panel has been optimized that includes CD45RO and CD25 in place of CD160 and CD137.
Conclusion:
See paper (https://www.ncbi.nlm.nih.gov/pubmed/27706900)
Notes:
See paper (https://www.ncbi.nlm.nih.gov/pubmed/27706900) See paper (https://www.ncbi.nlm.nih.gov/pubmed/27706900)