Long-term antibody response of CD46-IFNAR mice to immunization with MV-EDIII-ectoM.

aCD46-IFNAR mice were inoculated intraperitoneally (i.p.) twice with 104 TCID50 of MV-EDIII-ectoM at one month of interval. At 6 months, mice were boosted with 10 µg of recombinant DV1 rEDIII-ectoM protein (from S2 cells) in Alugel adjuvant. At 9 months, mice were inoculated with 107 FFU of DV-1 FGA/NA d1d. bDetermined by ELISA on pooled heat-inactivated sera collected one month after immunizations or DV infection. cFRNT50 represents the highest serum dilution that reduced the number of DV focus-forming units (FFU) on Vero cells by at least 50% and dFRNT75 by at least 75%.