E. coli Sacculi +A22 (MG1655)

Tilt Series Date: 2015-04-10 Data Taken By: Matt Swulius Species / Specimen: Escherichia coli Strain: MG1655 sacculi Tilt Series Settings: Single Axis, tilt range: (-60.0°, 60.0°), step: 1°, constant angular increment, dosage: 150.0 eV/Ų, defocus: -6.0 μm, magnification: 22500x. Microscope: Caltech Polara Acquisition Software: UCSFTomo Upload Method: pipeline Processing Software Used: raptor Sample Preparation: Cells were treated for one hour with 10ug/ml A22 until they were rounded and lemon-shaped prior to sacculus purification.

倾斜序列采集日期：2015-04-10
数据采集者：马特·斯怀尔斯（Matt Swulius）
物种/样品：大肠杆菌（Escherichia coli）
菌株：MG1655 细胞壁囊（sacculi）
倾斜序列采集参数：单轴倾斜模式，倾斜范围为(-60.0°, 60.0°)，步长1°，采用恒定角度增量；电子辐照剂量为150.0 eV/Ų，欠焦量为-6.0 μm，放大倍率为22500×。
所用显微镜：Caltech Polara型透射电子显微镜
采集软件：UCSFTomo
上传方式：流水线（pipeline）
所用数据处理软件：raptor
样品制备流程：在进行细胞壁囊纯化前，将细胞以10μg/ml浓度的A22处理1小时，直至细胞变为圆形并呈柠檬状形态。