PacBio HiFi sequencing of A. thaliana mutants of METHYLTRANSFERASE1 (MET1) individuals in Tsu-0 accession background

Because transposable elements (TEs) can cause heritable genetic changes, past work on TE mobility in Arabidopsis thaliana has mostly focused on new TE insertions in the germline of hypomethylated plants. It is, however, well-known that TEs can also be active in the soma, although the high-confidence detection of somatic events has been challenging. We leveraged the high accuracy of PacBio HiFi long reads to evaluate the somatic mobility of TEs in individuals of an A. thaliana non-reference strain lacking activity of METHYLTRANSFERASE1 (MET1), a major component of the DNA methylation maintenance machinery. Tsu-0 wild type and ten first-generation met1 mutants were independently sequenced with PacBio HiFi for assessing hypomethylation-dependent somatic transposition in single individuals. For all 11 samples, this project collects FASTQ files with HiFi reads generated with DeepConsensus, and BAM files with kinetics information necessary to analyze DNA methylation profiles. We also present the telomere-to-telomere assembly of accession Tsu-0 (ecotype ID: 7373).