Emx2 KO RNAseq

Emx2Cre/Cre vs. Emx2WT/WT by RNAseq Overall design: 10 utricles were dissected and pooled for each technical replicate and stored in RNAlater at 4C until genotypes were verified. RNA extraction was completed via Qiagen RNeasy micro Kit with DNAse I column digest for 4 technical replicates of each genotype. Concentrations were validated with Qubit and quality scores using Agilent High Sensitivity RNA ScreenTape Assay prior to RNA analysis. RNA library prep was completed using Illumina TruSeq Stranded Total RNA Library Prep Ribo-Zero Gold kit. Sequencing was completed with NovaSeq2 x 150bp Sequencing 200M read-pairs per sample. Subsequent alignments identified human RNA transcript read contamination in a single replicate, which was removed prior to DESeq2 analysis to identify upregulated and downregulated transcripts.