Tufted Puffin (Fratercula cirrhata) 3dRADseq and msat data

The tufted puffin (Fratercula cirrhata) is a medium sized seabird found throughout the North Pacific Ocean. Although populations of tufted puffins appear to be stable or increasing in some parts of their distribution, they are experiencing significant declines in other parts of their distribution, including in the Gulf of Alaska. Assessments of tufted puffin population status in Alaska are currently limited because little is known about population genetic structure. Similarly, there is little information on the amount of dispersal between populations in Alaska, and between populations in Alaska and those in the southern portion of the species’ range. If genetic analyses reveal significant genetic differentiation between tufted puffins nesting in different regions of Alaska, or evidence of local adaptation, then the apparent decline in the Gulf of Alaska becomes more of a conservation concern. There is general consensus within the tufted puffin research and management community that a st..., We isolated high quality genomic DNA from 75 egg, feather, tissue, and blood samples using a salt extraction (Aljanabi and Martinez 1997).  Samples were sent off to Université Laval where DNA was digested with three restriction enzymes PstI, NsiI, MspI and a library was prepared.  Sequencing was done at Génome Québec at McGill University on a NovaSeq6000 S4 PE100.,

簇羽海鹦（Fratercula cirrhata）是一种广泛分布于北太平洋全域的中型海鸟。尽管在部分分布区域，簇羽海鹦的种群数量看似稳定甚至有所增长，但在包括阿拉斯加湾在内的其他区域，该种群正经历显著的衰退。当前针对阿拉斯加地区簇羽海鹦种群现状的评估存在局限，原因是学界对其种群遗传结构的认知仍较为匮乏。同样，关于阿拉斯加境内各种群间，以及阿拉斯加种群与该物种分布范围南部种群间的扩散水平，现有相关信息也极为有限。若遗传分析显示，在阿拉斯加不同区域筑巢的簇羽海鹦之间存在显著遗传分化，或是存在本地适应的证据，那么阿拉斯加湾的种群衰退现象将成为更值得重视的保育关切。目前在簇羽海鹦的研究与管理界，学界已达成普遍共识：即一项未完成的表述（原文此处截断为"a st..."）。我们采用盐析法（Aljanabi与Martinez，1997）从75份卵、羽毛、组织及血液样本中提取了高质量基因组DNA。样本被送往拉瓦尔大学（Université Laval），在该校使用PstI、NsiI、MspI三种限制性内切酶对DNA进行酶切，并完成测序文库构建。测序工作由麦吉尔大学下属的魁北克基因组中心（Génome Québec）在NovaSeq6000 S4 PE100平台上完成。