Photoperiodic Transcriptome of Arabidopsis thaliana

Photoperiod is a circannual signal measured by biological systems to align growth and reproduction with the seasons. To understand the effect of photoperiod of gene expression in Arabidopsis thaliana in the absence of exogenous sugar under constant light intensity, we performed time course mRNA-seq analysis on 13-day old seedlings across three photoperiods with triplicates to identify photoperiod-regulated genes. Overall design: Time course bulk mRNA seq analysis of Arabidopsis seedling gene expression across three photoperiods: long day (16L:8D), equinox (12L:12D) and short day (8L:16D). Seedlings were grown without exogenous sugar for 10 days in equinox photoperiod before transfer to the respective photoperiod for 2 days. Whole seedlings were collected every four hours at Zeitgeber time 00 h, 04 h, 08 h, 12 h, 16 h and 20 h.