Spatial Ppara-Anxa2 Axis in Polyethyelen-Driven Liver Damage

Microplastics (MP) are increasingly recognized as an urgent global health concern, with mounting evidence linking their bioaccumulation to chronic disease risks. The liver, as a central organ for metabolic homeostasis and detoxification, is particularly sensitive to sustained MP exposure. However, the mechanisms underlying hepatic responses to polyethylene (PE), the most prevalent yet relatively understudied plastic type, remain poorly defined. Spatial transcriptomics uncovered the cell-type heterogeneity and gene expression patterns masked in bulk analyses. Spatial clustering uncovered inflammatory hotspots characterized by reduced cellular diversity and distinct PE-responsive gene signatures. This study provides a molecular framework for understanding MP-associated disruption of liver pathophysiology and highlights potential targets for therapeutic intervention. Here we share the shinyapp versions. 1) Mouse_MP_Polyethylene_Hepatocytes_Cholangiocytes https://ohbiolab.shinyapps.io/shinyApp_MP_Polyethylene_Hepatocytes/ 2) Mouse_MP_Polyethylene_NonParenchymal https://ohbiolab.shinyapps.io/shinyApp_MP_Polyethylene_NonParenchymal/ 3) Mouse_MP_Polyethyelen_downsize_20pct (for better loading speed) https://ohbiolab.shinyapps.io/shinyApp_MP_Polyethylene_downsize_20pct/微塑料（Microplastics, MP）正日益被视为亟待应对的全球性健康忧患，越来越多的研究证据表明其生物累积与慢性疾病风险存在显著关联。肝脏作为维持代谢稳态与执行解毒功能的核心器官，对持续的微塑料暴露尤为敏感。聚乙烯（Polyethylene, PE）是目前最为普遍但研究相对匮乏的塑料品类，但其诱导肝脏应答的具体分子机制仍尚未明确。空间转录组学（Spatial transcriptomics）能够揭示批量分析中被遮蔽的细胞类型异质性与基因表达特征。本研究通过空间聚类分析发现了以细胞多样性降低和独特的PE应答基因特征为标志的炎症热点区域。本研究为理解微塑料相关的肝脏病理生理紊乱提供了分子框架，并为治疗干预指明了潜在靶点。 本研究公开了三款Shiny应用工具： 1) 小鼠MP聚乙烯肝细胞与胆管上皮细胞（Mouse_MP_Polyethylene_Hepatocytes_Cholangiocytes） 访问链接：https://ohbiolab.shinyapps.io/shinyApp_MP_Polyethylene_Hepatocytes/ 2) 小鼠MP聚乙烯非实质细胞（Mouse_MP_Polyethylene_NonParenchymal） 访问链接：https://ohbiolab.shinyapps.io/shinyApp_MP_Polyethylene_NonParenchymal/ 3) 小鼠MP聚乙烯降采样20%版（Mouse_MP_Polyethyelen_downsize_20pct，用于优化加载速度） 访问链接：https://ohbiolab.shinyapps.io/shinyApp_MP_Polyethylene_downsize_20pct/