Effect on gene expression of addition of N-acetylmannosamine to galactose grown Streptococcus pneumoniae.

RNA-seq analysis of N-acetylmannosamine upon growth and gene expression in pneumococci grown with galactose as sole sugar. Pneumocccal strain D39 was grown to mid exponetial phase in chemically defined media (CDM) containing 55 mM galactose (as sole sugar). N-acetylmannosamine was added at 3.5 mM and samples were taken for RNA analysis at 0, 15 and 30 minutes post addition. Additionally separate D39 cultures were grown with 3.5 mM N-acetyl-mannosamine as the sole sugar and RNA extracted at mid-exponential.