Transcriptional analysis of transposon mutants disrupted in bacillithiol biosynthesis in Staphylococcus aureus

In this study we compare logrithmically grown Staphylococcus aureus SAUSA300 wildtype to a transposon mutant that is disrupted in bshC, the third step in bacillithiol biosynthesis using RNASeq to identify novel pathways where bacillithiol may be involved. Overall design: RNASeq analysis of wildtype cells and bshC:Tn grown logarithmically at 37C while shaken.