Cytoplasmic Location of Translation Controls Protein Output [CLIP-seq]. Cytoplasmic Location of Translation Controls Protein Output [CLIP-seq]

The cytoplasm contains membrane-bound organelles and membraneless compartments. The TIS granule network is formed through assembly of the RNA-binding protein TIS11B together with its bound mRNAs and associates with a portion of the rough endoplasmic reticulum (ER) – one of the major sites of protein synthesis. Subcellular mRNA localization is widespread in polarized cells. However, it is largely unknown if mRNA localization is also widespread in non-polarized cells and if the location of protein synthesis has biological consequences. To identify compartment-specific cellular functions, we used fluorescent particle sorting to isolate TIS granules, the ER surface, and the cytosol and determined their mRNA contents. Overall design: iCLIP-sequencing in HEK293T cells transfected with GFP-TIS11B, three biological replicates.