Gene expression-based, identification of differential expression genes in MDA-MB-231 cells with or without knockout of CCDC25 gene and transfected with NET-DNA

To explore the intracellular function of CCDC25, we transfected NET-DNA into MDA-MB-231 cells with or without CCDC25-knockout, and performed comparative microarray analyses. MDA-MB-231 cells transdecued with a control or sgRNA targeting CCDC25 were selected with puromycin for 2 weeks to get the stably transduced cells. 5 x 105 MDA-MB-231 with or without CCDC25 knockout were trasfected with 0.5 ug NET-DNA and cultured for 24 hours. The cells were isolated for further analyses and microarray