Elevated temperature during rearing diminishes swimming and disturbs the metabolism of yellow perch larvae

Temperate waters, such as the Great Lakes, are predicted to increase by 1°C every decade. Poikilothermic fish thermoregulate behaviourally, moving to more suitable thermal environments. Embryos are incapable of locomotion and may be exposed to non-optimal temperatures during development. Increased temperature alters the normal development of the yellow perch (Perca flavescens); however, whether altered incubation temperature influences the development of metabolism and function in these fish remains unknown. We hypothesized that increased embryonic incubation temperature would disturb cardiac and metabolic function and the behaviour of yellow perch larvae. We reared yellow perch embryos at 12°C, 15°C, or 18°C until hatching; after hatching, the temperature was raised to a common garden 18°C, their preferred post-hatch temperature. We assessed exploratory behaviour, metabolism (oxygen consumption), and cardiac performance throughout early development. At hatch, 12°C fish exhibited the gr..., Apoptosis stain
The acridine orange stain protocol for apoptotic cell death was adapted from Wong et al. (2021). At hatch, 20 yellow perch larvae were added to each well on a 6-well plate along with E2 fish water. After quick removal of fish water from each well, 3mL of fresh E2 at the appropriate incubation temperature or acridine orange solution (5 µg/ml in E2; Acridine orange hemi (zinc chloride) salt; Sigma-Aldrich; Cat No. A6014) was added for the control and stained groups, respectively. The plate was immediately covered and moved into a dark incubator at the respective temperature for 60 min. Post-incubation, embryos were washed with E2 at incubation temperatures. To measure whole-body fluorescence at hatch, stained and control fish were anesthetized with 0.016% MS-222 (Sigma Aldrich, Canada) in E2, and individual fish were placed into each well of a 96-well plate. The plate was read using the Synergy 2 Multi-Mode plate reader (BioTek Instruments, Winooski, United States) and Gen..., , # Data from: Elevated temperature during rearing diminishes swimming and disturbs the metabolism of yellow perch larvae

[https://doi.org/10.5061/dryad.c2fqz61m8](https://doi.org/10.5061/dryad.c2fqz61m8)

## Description of the data and file structure

**GENERAL INFORMATION**

This dataset is divided into folders according to the experiments from which the data was collected. We characterized cardiac development by assessing ejection fraction at hatch and heart rate at hatch and 20 days post-hatch (DPH). Whole-animal metabolic rates were measured at hatch, 5, 10, and 20 DPH. To probe into possible changes in mitochondrial respiration, we estimated basal metabolic rate, ATP production, proton leak, and non-mitochondrial respiration. To gauge locomotory function in larvae, we performed general swimming assessments from hatch until 5 DPH, and at 10 and 20 DPH. At hatch, transcript abundance was determined for vascular endothelial growth factor- A (vegfa), myosin heavy chain (myhc), and NKX2...,

温带水域（如五大湖）预计每十年升温1℃。变温鱼类（poikilothermic fish）会通过行为调节体温，迁移至更适宜的热环境中。而胚胎不具备运动能力，在发育过程中可能暴露于非最优温度环境。升温会改变黄鲈（*Perca flavescens*）的正常发育，但孵化温度变化是否会影响该鱼类的代谢与功能发育，目前仍不明确。我们提出假说：胚胎孵化期升温会干扰黄鲈幼体的心脏与代谢功能及行为表现。我们将黄鲈胚胎分别置于12℃、15℃或18℃环境中培育至孵化；孵化后，将所有幼体统一置于18℃（即黄鲈孵化后的偏好温度）的共养环境中。我们在早期发育阶段全程评估了其探索行为（exploratory behaviour）、代谢水平（耗氧量，oxygen consumption）与心脏功能（cardiac performance）。孵化时，12℃组幼体表现出…… 细胞凋亡染色
吖啶橙（Acridine orange）细胞凋亡染色方案改编自Wong等人（2021）的研究。孵化后，每孔6孔板中加入20尾黄鲈幼体与E2养殖水。快速移除每孔中的养殖水后，分别向对照组与染色组加入3mL对应孵化温度的新鲜E2培养基，或吖啶橙染色液（5 μg/mL溶于E2中；氯化锌半吖啶橙盐；Sigma-Aldrich；货号A6014）。随后立即封板并转移至对应温度的黑暗培养箱中孵育60分钟。孵育结束后，用对应孵化温度的E2培养基洗涤胚胎。为检测孵化时的全身体荧光强度，将染色组与对照组幼体用含0.016% MS-222（Sigma-Aldrich，加拿大）的E2培养基麻醉，随后将单尾幼体置于96孔板的每孔中。使用Synergy 2多功能酶标仪（BioTek Instruments，美国温诺斯基）与Gen……
# 数据来自：培育过程中的高温会削弱黄鲈幼体的游泳能力并干扰其代谢
[https://doi.org/10.5061/dryad.c2fqz61m8]
## 数据与文件结构说明
**基本信息**
本数据集按照数据采集的实验分组划分文件夹。我们通过检测孵化时的射血分数，以及孵化时与孵化后20天（DPH）的心率，来表征心脏发育情况。全机体代谢率分别在孵化时、孵化后5、10及20天进行测定。为探究线粒体呼吸可能发生的变化，我们估算了基础代谢率、ATP生成量、质子漏与非线粒体呼吸速率。为评估幼体的运动功能，我们分别在孵化至孵化后5天，以及孵化后10、20天开展了常规游泳能力评估。孵化时，我们对血管内皮生长因子-A（vascular endothelial growth factor-A，VEGFA）、肌球蛋白重链（myosin heavy chain，MYHC）以及NKX2……的转录本丰度进行了测定。