B2P 2024 Exometabolite incubation amplicon sequences

16s rRNA amplicon sequence files generated from the C-CoMP B2P BIOS Research Week 2024 exometabolite incubation experiment conducted at the Bermuda Institute of Ocean Sciences in March 2024. Sequence data files were generated from both natural, unamended seawater microbial communities (Bulk1_..., Bulk2_..., Bulk3_...) and experimental end-member communities, including: triplicate biotic (unamended) community DNA collected after 56 hours of incubation (B1_..., B2_..., B3_...), triplicate DNA samples from microbial communities amended with Micromonas commoda-derived metabolite mix after 56 hours (Mic1_..., Mic2_..., Mic3_...), and triplicate DNA samples from microbial communities amended with Prochlorococcus marinus-derived metabolite mix after 56 hours (Pro1_..., Pro2_..., Pro3_...). An average of 1800 mL of seawater was filtered to obtain DNA in the unamended seawater microbial communities, while an average of 800 mL seawater was filtered to obtain the end-member community DNA samples. The Dyhrman Lab extracted DNA, and library prep and sequencing were performed at Argonne National Laboratory on an Illumina MiSeq platform in May 2024. Code for bioinformatics analysis is stored at https://github.com/C-CoMP-STC/B2P23-BIOS-Week.本数据集为2024年3月于百慕大海洋科学研究所（Bermuda Institute of Ocean Sciences）开展的C-CoMP B2P BIOS 2024研究周胞外代谢物培养实验所产生的16S rRNA扩增子序列文件（16S rRNA amplicon sequence files）。测序数据源自两类样品：一是天然、未修饰的海水微生物群落（Bulk1_...、Bulk2_...、Bulk3_...），二是实验端元群落，具体包括：经56小时培养后采集的三份重复未修饰微生物群落DNA样本（B1_...、B2_...、B3_...）；经56小时培养、添加了小定鞭金藻（Micromonas commoda）来源代谢物混合物的微生物群落的三份重复DNA样本（Mic1_...、Mic2_...、Mic3_...）；以及经56小时培养、添加了海洋原绿球藻（Prochlorococcus marinus）来源代谢物混合物的微生物群落的三份重复DNA样本（Pro1_...、Pro2_...、Pro3_...）。天然未修饰海水微生物群落的DNA提取过程中，平均过滤海水体积为1800 mL；而端元群落DNA样本的提取则平均过滤800 mL海水。DNA提取由Dyhrman实验室（Dyhrman Lab）完成，文库制备与测序于2024年5月在阿尔贡国家实验室（Argonne National Laboratory）的Illumina MiSeq测序平台（Illumina MiSeq）上开展。生物信息学分析所用代码已存储于https://github.com/C-CoMP-STC/B2P23-BIOS-Week。