Time-course transcriptome analysis in roots of N-limited Medicago truncatula plants grown in split-root inoculated with Sinorhizobium medicae. Time-course transcriptome analysis in roots of N-limited Medicago truncatula plants grown in split-root inoculated with Sinorhizobium medicae

The experiment aims to analyze the effect of inoculation with Sinorhizobium medicae MD4 of on the transcriptome of the Medicago truncatula roots of N-limited plants. We divided the roots systems into two parts; one of them was supplied with nutrient solution with mineral N (0.5 mM for N-limited plants) whereas the non-N treated part was kept in nutrient solution without mineral N. All roots were inoculated 48h after initiation of the N treatments. We collected the roots the non-N treated side of the split roots system and we isolated total RNA for RNAseq analysis. Root transcriptomes were analyze before inoculation, at 1 and 2 days post-inoculation.