Becoming LTi - Dataset : Single-cell RNA-seq of Embryo Fetal Liver tissue at stage 14.5 days

<strong>Dataset from article</strong> : Distinct waves from the hemogenic endothelium give rise to layered Lymphoid Tissue Inducer cell ontogeny <strong>Summary:</strong> During embryogenesis Lymphoid Tissue Inducer (LTi) cells are essential for lymph node organogenesis. These cells are part of the Innate Lymphoid Cell (ILC) family. Although their earliest embryonic hematopoietic origin is unclear, other innate immune cells were shown to be derived from both early hemogenic endothelium in the yolk-sac as well as the aorta-gonad-mesonephros. A proper model to discriminate between these locations was unavailable. In this study, using a new Cxcr4-CreERT2 lineage tracing model, we identify a major contribution from embryonic hemogenic endothelium, but not yolk-sac, towards the LTi progenitors. Conversely, embryonic LTi cells are replaced by hematopoietic stem cell derived cells in adult. We further show that within the fetal liver common lymphoid progenitors differentiate into highly dynamic alpha-lymphoid precursor cells, which at this embryonic stage preferentially mature into LTi precursors and establish their functional LTi cell identity only after reaching the periphery. <strong>Data </strong>: 1. SPlab_BecomingLTi_Stage14.5_FetalLiver_00_RawData : Single-cell RNA-seq data for Mouse Embryo Fetal Liver at stage 14.5. It contains the full output of CellRanger count (v3) analysis.**论文来源数据集**：生血内皮细胞的不同发育波次介导分层式淋巴组织诱导细胞个体发生 **摘要**：胚胎发育过程中，淋巴组织诱导细胞（Lymphoid Tissue Inducer, 简称LTi）是淋巴结器官发生的关键细胞类群，隶属于固有淋巴样细胞（Innate Lymphoid Cell, 简称ILC）家族。尽管其最早的胚胎造血起源尚未明确，但已有研究证实其他固有免疫细胞同时来源于卵黄囊（yolk-sac）中的早期生血内皮细胞以及主动脉-性腺-中肾区（aorta-gonad-mesonephros）。此前尚无能够区分这两类起源位点的可靠实验模型。本研究借助全新的Cxcr4-CreERT2谱系示踪模型，证实胚胎生血内皮细胞（而非卵黄囊）对LTi祖细胞存在主要贡献。与之相反，成体体内的胚胎源性LTi细胞会被造血干细胞衍生的细胞所替代。本研究还进一步发现，在胎肝中，共同淋巴样祖细胞会分化为具有高度动态特性的α淋巴样前体细胞（alpha-lymphoid precursor cells）；在该胚胎发育阶段，这类前体细胞优先成熟为LTi祖细胞，并仅在迁移至外周组织后才确立其功能性LTi细胞身份。 **数据集**： 1. SPlab_BecomingLTi_Stage14.5_FetalLiver_00_RawData：针对14.5天小鼠胚胎胎肝的单细胞RNA测序（single-cell RNA-seq）数据，包含CellRanger计数（v3）分析的全部输出结果。