Major VACV strains used in this study.

1All strains were generated in the Western Reserve (WR) strain of VACV. 2“+” indicates locus is intact and “−” indicates locus is disrupted. Marker genes and inserted viral or human genes present at disrupted loci are in parentheses. Abbreviations: His, His6 epitope tag; Flag, Flag epitope tag; R1BD, R1-binding domain; VACV, vaccinia virus; ECTV, ectromelia virus; MYX, myxoma virus; SFV, Shope fibroma virus; HR1, human R1; Hp53R2, human p53R2. See Materials and Methods and Text S1 for further details.