Binding parameters predicted by software Oligo walk 5.0 and PNALIGHT.
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aNumbering from the first base of the gene rpoD;bΔG means free energy; index for each parameter: GC%≤60%, overall ΔG50°C;c“—” means not determined;dCalculated melting temperature in [°C] of matching PNA/DNA hybrids with no dangling ends.
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2015-12-02



