CBMN assessment of carotenoids in HepG2 cells

This dataset contains micrographs of HepG2 cells exposed for 24h to the following carotenoids: 4.6 µM β-carotene, 40 µM trans-β-apo-8’-carotenal and 10 µM β-ionone 10 µM. Controls included cells grown only in culture medium, cells grown in solvent control (0.5% acetone) and cells grown in positive control 20 µM methyl methanesulfonate (MMS), a mutagenic compound. Cells were further incubated with cytochalalin B for a sufficient period of time to allow nuclear division while preventing cytokinesis.

本数据集包含经下述类胡萝卜素（carotenoids）处理24小时的HepG2细胞（HepG2 cells）的显微图像：4.6 μM β-胡萝卜素（β-carotene）、40 μM 反式-β-阿朴-8’-胡萝卜素醛（trans-β-apo-8’-carotenal）及10 μM β-紫罗兰酮（β-ionone）。对照组包括仅以细胞培养液培养的空白细胞、以0.5%丙酮作为溶剂对照培养的细胞，以及以20 μM 甲磺酸甲酯（methyl methanesulfonate，MMS，一种诱变化合物）作为阳性对照培养的细胞。后续将细胞与细胞松弛素B（cytochalasin B，原文作cytochalalin B）共同孵育足够时长，使其可发生核分裂同时阻断胞质分裂。